Review



klenow  (New England Biolabs)


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    Structured Review

    New England Biolabs klenow
    Klenow, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/klenow+fragment/Klenow+Fragment/pmc13171454-66-18-20
    Average 99 stars, based on 6889 article reviews
    klenow - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: BCS2.0: a capture sequencing platform for rapid differential diagnosis of bacterial infections and antimicrobial resistance
    Article Snippet: Nucleic acid quantity and quality were assessed using a NanoDrop (ThermoFisher) and a Qubit (ThermoFisher). .. TNA was reverse transcribed to cDNA using Superscript IV (ThermoFisher) and Klenow fragment (NEB). cDNA was quantified using Qubit prior to enzymatic fragmentation. ..

    Enzymatic Fragmentation:

    Article Title: BCS2.0: a capture sequencing platform for rapid differential diagnosis of bacterial infections and antimicrobial resistance
    Article Snippet: Nucleic acid quantity and quality were assessed using a NanoDrop (ThermoFisher) and a Qubit (ThermoFisher). .. TNA was reverse transcribed to cDNA using Superscript IV (ThermoFisher) and Klenow fragment (NEB). cDNA was quantified using Qubit prior to enzymatic fragmentation. ..

    Hi-C:

    Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
    Article Snippet: .. A-tailing of Hi-C DNA was performed by adding 2.5 μL of 10X NEB2 buffer (B7002S), 5 μL of 1 mM dATP (N0440S), and 1.5 μL of Klenow fragment (NEB, M0212L) to the 16 μL of resuspended streptavidin beads. ..

    Purification:

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Phospho-proteomics:

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Ligation:

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Concentration Assay:

    Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA
    Article Snippet: .. Adapters were annealed at a final concentration of 2.5 μM in the same way (o4371 + o4372, o4373 + o4374, and o4392 + o4393) and subsequently extended with 25 mU/μl Klenow fragment (NEB) in the presence of 0.1 mM dNTPs for 20 min at 37°C. ..



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